Anti-MBD1抗体- ChIP Grade (ab2846)


  • 产品名称
    Anti-MBD1抗体- ChIP Grade
    参阅全部 MBD1 一抗
  • 描述
    兔多克隆抗体to MBD1 - ChIP Grade
  • 特异性
    This antibody detects human methyl CpG binding domain protein 1 (MBD1) recombinant protein.
  • 经测试应用
    适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human MBD1 aa 593-605.


    (Peptide available as ab4918)



Our Abpromise guarantee covers the use of ab2846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 5 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).Can be blocked with MBD1 peptide (ab4918).
ChIP 1/50.


  • 功能
    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Contains 3 CXXC-type zinc fingers.
    Contains 1 MBD (methyl-CpG-binding) domain.
  • 结构域
    The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions.
    The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides.
    The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
  • 翻译后修饰
    Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CXXC 3 antibody
    • CXXC-type zinc finger protein 3 antibody
    • CXXC3 antibody
    • MBD 1 antibody
    • Mbd1 antibody
    • MBD1_HUMAN antibody
    • MECP1 COMPLEX antibody
    • Methyl CpG binding domain protein 1 antibody
    • Methyl CpG binding domain protein 1 isoform PCM1 antibody
    • Methyl CpG binding protein MBD1 antibody
    • Methyl CpG binding protein splice variant 1 antibody
    • Methyl CpG binding protein splice variant 2 antibody
    • Methyl CpG binding protein splice variant 3 antibody
    • Methyl CpG binding protein splice variant 4 antibody
    • Methyl-CpG-binding domain protein 1 antibody
    • Methyl-CpG-binding protein MBD1 antibody
    • PCM 1 antibody
    • PCM1 antibody
    • Protein containing methyl-CpG-binding domain 1 antibody
    • Regulator of fibroblast growth factor 2 (FGF 2) transcription antibody
    • RFT antibody
    • The regulator of fibroblast growth factor 2(FGF 2) transcription antibody
    see all


  • Predicted band size : 83 kDa
    Western blot showing approx 83 kD band, representing recombinant human MBD 1


This product has been referenced in:
  • Laget S  et al. MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress. Epigenetics 9:546-56 (2014). Read more (PubMed: 24434851) »
  • Katryniok C  et al. Role of DNA methylation and methyl-DNA binding proteins in the repression of 5-lipoxygenase promoter activity. Biochim Biophys Acta 1801:49-57 (2010). WB, ChIP ; Human . Read more (PubMed: 19781662) »

See all 4 Publications for this product


Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - nuclear (Breast Cancer Cell Line MCF-7)
Breast Cancer Cell Line MCF-7
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
Amplification of sonicated genomic DNA (gDNA) with gene-specific primers prior immunoprecipitation served as positive control.
Negative control
DNA immunoprecipitated with the antibody IgG served as negative control

Dr. Heidi Schwarzenbach

Verified customer

提交于 May 01 2007

Thank you for your enquiry and for your patience. I have been looking into the issue that you have raised. Both ab3753 and ab2846 are originated outside Abcam and by two different sources. The originator of ab2846 said that all evidence that th...

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