The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ChIP: Use at an assay dependent dilution (PMID 15327775).
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
结构域The methyl-CpG-binding domain (MBD) functions both in binding to methylated DNA and in protein interactions. The third CXXC-type zinc finger mediates binding to non-methylated CpG dinucleotides. The transcriptional repression domain (TRD) is involved in transcription repression and in protein interactions.
翻译后修饰Sumoylated with SUMO1 by PIAS1 and PIAS3. Sumoylation affects transcriptional silencing by preventing the interaction with SETDB1. In contrast, sumoylation may increase interaction with AFT7IP. Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus speckle. Chromosome. Nuclear, in a punctate pattern. Associated with euchromatic regions of the chromosomes, with pericentromeric regions on chromosome 1 and with telomeric regions from several chromosomes.
Sarraf SA & Stancheva I Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Mol Cell15:595-605 (2004).
Read more (PubMed: 15327775) »
Reese BE et al. The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1. Mol Cell Biol23:3226-36 (2003).
Read more (PubMed: 12697822) »