概述

  • 产品名称
  • 描述
    兔多克隆抗体to MAZ
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Pig, Chimpanzee, Rhesus monkey, Chinese hamster
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 427-477 of Human MAZ, BAA33064.1

  • 阳性对照
    • HeLa cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May function as a transcription factor with dual roles in transcription initiation and termination. Binds to two sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.
  • 组织特异性
    Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex and midfrontal cortex (at protein level).
  • 序列相似性
    Contains 6 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus. In brains of Alzheimer disease patients, present in a plaque-like structures.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAZ antibody
    • MAZ_HUMAN antibody
    • MAZI antibody
    • MYC associated zinc finger protein antibody
    • Myc-associated zinc finger protein antibody
    • Pur-1 antibody
    • Pur1 antibody
    • Purine binding transcription factor antibody
    • Purine-binding transcription factor antibody
    • SAF 1 antibody
    • SAF 2 antibody
    • SAF-1 antibody
    • SAF-2 antibody
    • SAF-3 antibody
    • Serum amyloid A activating factor 1 antibody
    • Serum amyloid A activating factor 2 antibody
    • Transcription factor Zif87 antibody
    • ZF87 antibody
    • Zif87 antibody
    • Zinc finger protein 801 antibody
    • Zinc finger protein, 87 kilodaltons antibody
    • ZNF801 antibody
    see all

Anti-MAZ antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling MAZ with ab85725 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MAZ antibody (ab85725) at 0.1 µg/ml

    Lane 1 : HeLa cells lysate at 50 µg
    Lane 2 : HeLa cells lysate at 15 µg
    Lane 3 : HeLa cells lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,39 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human MAZ by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded) using ab85725 at 3 ug/mg lysate from HeLa cells (lane 1). Lane 2 is Control IgG IP. Subsequent WB detection was performed using 1 ug/ml ab85725. Detection: Chemiluminescence with exposure times of 30 seconds.

Anti-MAZ antibody (ab85725)参考文献

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