概述

  • 产品名称Anti-MAZ抗体
    参阅全部 MAZ 一抗
  • 描述
    兔多克隆抗体to MAZ
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide within residues: FPVFPCTLLA PPFPVLGLDS RGVGGLMNSF PPPQGHAQNP LQVGAELQSR , corresponding to N terminal amino acids 2-51 of Human MAZ (NP_002374)

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab83397 is purified by a peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use at an assay dependent concentration. PubMed: 22415301
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May function as a transcription factor with dual roles in transcription initiation and termination. Binds to two sites, ME1a1 and ME1a2, within the MYC promoter having greater affinity for the former. Also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors.
  • 组织特异性Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex and midfrontal cortex (at protein level).
  • 序列相似性Contains 6 C2H2-type zinc fingers.
  • 细胞定位Nucleus. In brains of Alzheimer disease patients, present in a plaque-like structures.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAZ antibody
    • MAZ_HUMAN antibody
    • MAZI antibody
    • MYC associated zinc finger protein antibody
    • Myc-associated zinc finger protein antibody
    • Pur-1 antibody
    • Pur1 antibody
    • Purine binding transcription factor antibody
    • Purine-binding transcription factor antibody
    • SAF 1 antibody
    • SAF 2 antibody
    • SAF-1 antibody
    • SAF-2 antibody
    • SAF-3 antibody
    • Serum amyloid A activating factor 2 antibody
    • Transcription factor Zif87 antibody
    • ZF87 antibody
    • Zif87 antibody
    • Zinc finger protein 801 antibody
    • ZNF801 antibody
    see all

Anti-MAZ antibody 图像

  • Anti-MAZ antibody (ab83397) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling MAZ with ab83397 at 10µg/ml.

Anti-MAZ antibody (ab83397)参考文献

This product has been referenced in:
  • Smits M  et al. Myc-associated zinc finger protein (MAZ) is regulated by miR-125b and mediates VEGF-induced angiogenesis in glioblastoma. FASEB J 26:2639-47 (2012). IHC-P ; Human . Read more (PubMed: 22415301) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry regarding ab83397.
As the datasheet indicates the concentration of this antibody is 1 mg/ml and the unit size is 50 µg.
I hope this helps and if I can assist further, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"