Anti-Maxi Potassium channel alpha抗体(ab3586)

概述

  • 产品名称
    Anti-Maxi Potassium channel alpha抗体
    参阅全部 Maxi Potassium channel alpha 一抗
  • 描述
    兔多克隆抗体to Maxi Potassium channel alpha
  • 特异性
    Detects Maxi K+ alpha from Human tissues as well as recombinant Human protein.
  • 经测试应用
    适用于: ICC, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Horse, Human, Pig
    预测可用于: Rabbit, Chicken, Cow, Dog, Caenorhabditis elegans, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Maxi Potassium channel alpha aa 945-961.
    Sequence:

    TELVNDTNVQFLDQDDD


    (Peptide available as ab5022)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 2 µg/ml. Can be blocked with Maxi Potassium channel alpha peptide (ab5022).
IHC-P 1/200.

靶标

  • 功能
    Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
  • 组织特异性
    Widely expressed. Except in myocytes, it is almost ubiquitously expressed.
  • 疾病相关
    Generalized epilepsy and paroxysmal dyskinesia
  • 序列相似性
    Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily.
    Contains 1 RCK N-terminal domain.
  • 结构域
    The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4.
    The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
    The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.
    The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+).
    The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel.
    The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation.
  • 翻译后修饰
    Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.
    Palmitoylation by ZDHHC22 and ZDHHC23 within the intracellular linker between the S0 and S1 transmembrane domains regulates localization to the plasma membrane. Depalmitoylated by LYPLA1 and LYPLAL1, leading to retard exit from the trans-Golgi network.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • subfamily M subunit alpha-1 antibody
    • BK channel antibody
    • BKCA alpha antibody
    • BKCA alpha subunit antibody
    • BKTM antibody
    • Calcium-activated potassium channel antibody
    • Calcium-activated potassium channel subunit alpha-1 antibody
    • Drosophila slowpoke like antibody
    • hSlo antibody
    • K(VCA)alpha antibody
    • KCa1.1 antibody
    • KCMA1_HUMAN antibody
    • KCNMA antibody
    • KCNMA1 antibody
    • Maxi K channel antibody
    • Maxi Potassium channel alpha antibody
    • MaxiK antibody
    • SAKCA antibody
    • SLO alpha antibody
    • SLO antibody
    • Slo homolog antibody
    • Slo-alpha antibody
    • Slo1 antibody
    • Slowpoke homolog antibody
    see all

文献

This product has been referenced in:
  • Mobasheri A  et al. Characterization of a stretch-activated potassium channel in chondrocytes. J Cell Physiol 223:511-8 (2010). IHC-P ; Horse . Read more (PubMed: 20162564) »
  • Floyd RV  et al. Morphology, calcium signaling and mechanical activity in human ureter. J Urol 180:398-405 (2008). Human . Read more (PubMed: 18495171) »

See all 2 Publications for this product

客户评价及客户问答

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Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (8% gel)
Sample
Pig Cell lysate - whole cell (smooth muscle cell)
Specification
smooth muscle cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Miss. Anna Sun

Verified customer

提交于 Aug 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Cartilage, articular)
Specification
Cartilage, articular
Fixative
Neutral buffered formalin
Antigen retrieval step
None
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

提交于 Feb 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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