概述

  • 产品名称Anti-MAVS抗体- ChIP Grade
    参阅全部 MAVS 一抗
  • 描述
    兔多克隆抗体to MAVS - ChIP Grade
  • 经测试应用适用于: IHC-P, ChIP, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from within residues 100 - 200 of Human MAVS.

    (Peptide available as ab25852.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat. A431. This antibody gave a positive signal in the following Formaldehyde fixed cell line: HEK 293.

性能

应用

Our Abpromise guarantee covers the use of ab31334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57, 75 kDa (predicted molecular weight: 57 kDa).
IP Use at an assay dependent concentration. PubMed: 21068253

靶标

  • 功能Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis.
  • 组织特异性Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
  • 序列相似性Contains 1 CARD domain.
  • 结构域Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1.
  • 翻译后修饰Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation.
  • 细胞定位Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CARD adapter inducing interferon beta antibody
    • CARD adaptor inducing IFN beta antibody
    • Cardif antibody
    • DKFZp666M015 antibody
    • FLJ27482 antibody
    • FLJ41962 antibody
    • IFN B promoter stimulator 1 antibody
    • Interferon beta promoter stimulator protein 1 antibody
    • Ips 1 antibody
    • IPS-1 antibody
    • Ips1 antibody
    • KIAA1271 antibody
    • MAVS antibody
    • MAVS_HUMAN antibody
    • Mitochondrial anti viral signaling protein antibody
    • Mitochondrial Antiviral Signaling antibody
    • Mitochondrial antiviral signaling protein antibody
    • Mitochondrial antiviral-signaling protein antibody
    • Putative NF kappa B activating protein 031N antibody
    • Putative NF-kappa-B-activating protein 031N antibody
    • Virus induced signaling adapter antibody
    • virus induced signaling adaptor antibody
    • Virus-induced-signaling adapter antibody
    • VISA antibody
    see all

Anti-MAVS antibody - ChIP Grade 图像

  • All lanes : Anti-MAVS antibody - ChIP Grade (ab31334) at 1 µg/ml

    Lane 1 : Hela Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57,75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 51 kDa (possible non-specific binding).

    The bands at 75 and 57 kDa correspond to the bands seen in a paper by Seth et al., 2005 (See Supplementary Information). PubMed: 16125763. The band at 51 kDa may represent non-specific antibody binding.

  • ICC/IF image of ab31334 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31334, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab31334 staining MAVS in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% NGS, 5% BSA in PBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in diluent) for 16 hours at 4°C. A Goat anti-rabbit HRP polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • MAVS was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal MAVS and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31334.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 75kDa: MAVS

Anti-MAVS antibody - ChIP Grade (ab31334)参考文献

This product has been referenced in:
  • Yuan D  et al. Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs. Oncotarget 6:29664-74 (2015). WB . Read more (PubMed: 26336994) »
  • Xia M  et al. Mitophagy enhances oncolytic measles virus replication by mitigating DDX58/RIG-I-like receptor signaling. J Virol 88:5152-64 (2014). Read more (PubMed: 24574393) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium Cytrat
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

提交于 Oct 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (melanoma)
Specification melanoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Angela Smilansy

Verified customer

提交于 Oct 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 30 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

ilan sela

Verified customer

提交于 Aug 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (T-REX)
Loading amount 20 µg
Specification T-REX
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

ilan sela

Verified customer

提交于 May 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium citrate
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

ilan sela

Verified customer

提交于 May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"