Anti-Matrix protein 1抗体[GA2B] (ab22396)

概述

  • 产品名称Anti-Matrix protein 1抗体[GA2B]
    参阅全部 Matrix protein 1 一抗
  • 描述
    小鼠单克隆抗体[GA2B] to Matrix protein 1
  • 经测试应用适用于: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    Reacts with Influenza A. Not yet tested in other species.
  • 免疫原

    Tissue/ cell preparation- Influenza A/ Puerto Rico/ 8/ 34 (H1N1) and A/Bangkok/ 1/ 79 (H3N2) viruses

性能

应用

Our Abpromise guarantee covers the use of ab22396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 20413723ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF 1/100.

靶标

  • 相关性Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions.
  • 细胞定位Cytoplasmic
  • 别名
    • M1 antibody
    • Matrix Protein 1 antibody
    • Matrix Protein I antibody
    • Membrane matrix protein M1 antibody

Anti-Matrix protein 1 antibody [GA2B] 图像

  • ab22396 used in Flow Cytometry.
    Influenza infected A549 cells were trypsinized, fixed in paraformaldehyde, permeabilized and then incubated with ab22396 for analysis by Flow Cytometry.

    ab22396 used at a 1/200 dilution.
    The secondary used was ab96874, diluted 1/200.

    See Abreview

  • Immunofluorescence analysis of HeLa cells staining Influenza A Virus M1 using ab22396.

    Cells were treated with either 20 µg/ml Prostaglandin A (PGA) or EtOH vehicle control, 3 hours post infection by Influenza A Virus, then fractionated at 9 hours post infection before analysis by immunofluorescence.

Anti-Matrix protein 1 antibody [GA2B] (ab22396)参考文献

This product has been referenced in:
  • Thompson CM  et al. Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems. BMC Biotechnol 15:31 (2015). WB ; Human . Read more (PubMed: 25981500) »
  • Krol E  et al. Anti-influenza A virus activity of uridine derivatives of 2-deoxy sugars. Antiviral Res 100:90-97 (2013). Read more (PubMed: 23911991) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Influenza infected A549)
Specification Influenza infected A549
Preparation Cell harvesting/tissue preparation method: Trypsinised
Sample buffer: PBS + 1%BSA + EDTA
Fixation Paraformaldehyde
Permeabilization Yes - BD Fix/Perm Kit
Gating Strategy Single cell suspension
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提交于 Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"