Anti-Matrix蛋白1抗体[GA2B] (ab22396)
Key features and details
- Mouse monoclonal [GA2B] to Matrix protein 1
- Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG1
概述
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产品名称
Anti-Matrix蛋白1抗体[GA2B]
参阅全部 Matrix protein 1 一抗 -
描述
小鼠单克隆抗体[GA2B] to Matrix蛋白1 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Influenza A -
免疫原
Tissue, cells or virus corresponding to Matrix protein 1. Influenza A/ Puerto Rico/ 8/ 34 (H1N1) and A/Bangkok/ 1/ 79 (H3N2) viruses
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
SDS-PAGE -
纯化说明
>90% IgG content as established by SDS PAGE -
克隆
单克隆 -
克隆编号
GA2B -
骨髓瘤
P3x63-Ag8.653 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22396于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt | (1) |
Use at an assay dependent concentration. PubMed: 20413723
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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ICC/IF |
1/100.
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说明 |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 20413723 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
1/100. |
靶标
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相关性
Influenza virus type A matrix protein, also known as M1, is composed of a 252 amino acid sequence and is type-specific in influenza viruses. It is located inside the viral lipid envelope and plays a key role in virus assembly and replication. M1 can be isolated from particles by removing the envelope with detergents and reducing the pH to 4.0. Influenza viruses are a common and widely spread infectious agent. Like many other viruses, influenza virus are constantly undergoing mutations and thereby avoiding the immune system. The Influenza A Virus M proteins form a continuous shell on the inner side of the lipid bilayer, maintaining the structural integrity of the virus particle through hydrophobic interactions. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 1461001 Influenza A
- Entrez Gene: 956527 Influenza A
- SwissProt: P03485 Influenza A
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别名
- M1 antibody
- Matrix Protein 1 antibody
- Matrix Protein I antibody
- Membrane matrix protein M1 antibody
图片
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Immunofluorescence analysis of HeLa cells staining Influenza A Virus M1 using ab22396.
Cells were treated with either 20 µg/ml Prostaglandin A (PGA) or EtOH vehicle control, 3 hours post infection by Influenza A Virus, then fractionated at 9 hours post infection before analysis by immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab22396 被引用在 24 文献中.
- Gromadzka B et al. Characterization of Immune Response towards Generation of Universal Anti-HA-Stalk Antibodies after Immunization of Broiler Hens with Triple H5N1/NA-HA-M1 VLPs. Viruses 14:N/A (2022). PubMed: 35458460
- Kim KH et al. Universal protection against influenza viruses by multi-subtype neuraminidase and M2 ectodomain virus-like particle. PLoS Pathog 18:e1010755 (2022). PubMed: 36006890
- Kang T et al. Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infection. Nat Commun 13:5841 (2022). PubMed: 36192426
- Mallampalli RK et al. Interferon Lambda Signaling in Macrophages Is Necessary for the Antiviral Response to Influenza. Front Immunol 12:735576 (2021). PubMed: 34899695
- Amarelle L et al. Cardiac glycosides decrease influenza virus replication by inhibiting cell protein translational machinery. Am J Physiol Lung Cell Mol Physiol 316:L1094-L1106 (2019). PubMed: 30892074