Anti-Matrin 3抗体(ab84422)
Key features and details
- Rabbit polyclonal to Matrin 3
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Matrin 3抗体
参阅全部 Matrin 3 一抗 -
描述
兔多克隆抗体to Matrin 3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Bat -
免疫原
Synthetic peptide designed within residues: GFYCKLCSLF YTNEEVAKNT HCSSLPHYQK LKKFLNKLAE ERRQKKET, corresponding to C terminal amino acids 800-847 of Human Matrin 3 (NP_061322.2)
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阳性对照
- IHC: Human: breast carcinoma, colon adenocarcinoma. Mouse: squamous cell carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84422于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: expected to work on frozen sections.
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ICC/IF | (2) |
1/100.
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说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Note: expected to work on frozen sections. |
ICC/IF
1/100. |
靶标
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功能
May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. -
疾病相关
Defects in MATR3 are the cause of myopathy distal type 2 (MPD2) [MIM:606070]; also called vocal cord and pharyngeal dysfunction with distal myopathy (VCPDM). MPD2 is a muscular disorder characterized by distal weakness, with onset in hands and feet, associated with vocal cord and pharyngeal weakness causing a nasal voice and swallowing disorders. -
序列相似性
Contains 1 matrin-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
细胞定位
Nucleus matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 9782 Human
- Entrez Gene: 17184 Mouse
- Omim: 164015 Human
- SwissProt: P43243 Human
- SwissProt: Q8K310 Mouse
- Unigene: 268939 Human
- Unigene: 595110 Human
- Unigene: 215034 Mouse
see all -
别名
- ALS21 antibody
- KIAA0723 antibody
- Matr3 antibody
see all
图片
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma using 1/250 ab84422, followed by DAB staining.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse squamous cell carcinoma using 1/250 ab84422, followed by DAB staining.
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Detection of Human Matrin 3 by Immunofluorescence.
Sample: FFPE section of human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500. -
Detection of Human Matrin 3 by Immunofluorescence.
Sample: FFPE section of Human breast carcinoma.Antibody: ab84422 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500. -
ab84422 staining Matrin 3 in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.5% NP-40, blocked with 1% BSA for 1 hour a room temperature, then incubated with ab84422 at a 1/100 dilution for 1 hour. The secondary antibody used was an Alexa-Fluor 488 conjugate donkey polyclonal, used at a 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab84422 被引用在 6 文献中.
- You J et al. Selective Loss of MATR3 in Spinal Interneurons, Upper Motor Neurons and Hippocampal CA1 Neurons in a MATR3 S85C Knock-In Mouse Model of Amyotrophic Lateral Sclerosis. Biology (Basel) 11:N/A (2022). PubMed: 35205163
- Cha HJ et al. Inner nuclear protein Matrin-3 coordinates cell differentiation by stabilizing chromatin architecture. Nat Commun 12:6241 (2021). PubMed: 34716321
- Kao CS et al. Selective neuronal degeneration in MATR3 S85C knock-in mouse model of early-stage ALS. Nat Commun 11:5304 (2020). PubMed: 33082323
- Ramesh N et al. RNA dependent suppression of C9orf72 ALS/FTD associated neurodegeneration by Matrin-3. Acta Neuropathol Commun 8:177 (2020). PubMed: 33129345
- Zhao M et al. Knockdown of genes involved in axonal transport enhances the toxicity of human neuromuscular disease-linked MATR3 mutations in Drosophila. FEBS Lett 594:2800-2818 (2020). PubMed: 32515490
- Rajgor D et al. Identification of novel nesprin-1 binding partners and cytoplasmic matrin-3 in processing bodies. Mol Biol Cell 27:3894-3902 (2016). WB, ICC/IF ; Human . PubMed: 27733621