概述

  • 产品名称Anti-Matrilin 4抗体
    参阅全部 Matrilin 4 一抗
  • 描述
    兔多克隆抗体to Matrilin 4
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 13 amino acid synthetic peptide near the N-terminus of Human Matrilin 4 (NP_085080).

  • 阳性对照
    • Rat brain tissue lysate; Mouse brain tissue IF/ICC: A498 cell line.

性能

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应用

Our Abpromise guarantee covers the use of ab106379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: Isoform 1, 68 ; Isoform 4, 59 kDa.
IHC-P Use at an assay dependent concentration.

靶标

Anti-Matrilin 4 antibody 图像

  • ab106379 stained A498 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106379 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature

  • Immunofluorescence of Matrilin 4 in mouse brain tissue with ab106379 at 20 ug/mL.

  • All lanes : Anti-Matrilin 4 antibody (ab106379) at 1 µg/ml

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : Isoform 1, 68 ; Isoform 4, 59 kDa
  • ab106379, at 2.5 µg/ml, staining Matrilin 4 in Mouse brain tissue by Immunohistochemistry.
  • Immunohistochemical analysis of Human cornea tissue, staining Matrilin 4 wih ab106379.

    Tissue was taken from healthy patients (left) or patients with granular dystrophy (right). Sections were blocked with blocking solution for 30 minutes at room temperature before incubation with primary antibody (1/300) overnight at 4°C. Staining was detected with DAB.

Anti-Matrilin 4 antibody (ab106379)参考文献

This product has been referenced in:
  • Szalai E  et al. Fibrillin-2, tenascin-C, matrilin-2, and matrilin-4 are strongly expressed in the epithelium of human granular and lattice type I corneal dystrophies. Mol Vis 18:1927-36 (2012). IHC-P ; Human . Read more (PubMed: 22876117) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"