ab106379 stained A498 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106379 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature
ab106379, at 2.5 µg/ml, staining Matrilin 4 in Mouse brain tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Matrilin 4 antibody (ab106379)Image from Szalai E et al., Mol Vis. 2012;18:1927-36. Epub 2012 Jul 18. Fig 5.; 18 July 2012, Molecular Vision 2012; 18:1927-1936
Immunohistochemical analysis of Human cornea tissue, staining Matrilin 4 wih ab106379.
Tissue was taken from healthy patients (left) or patients with granular dystrophy (right). Sections were blocked with blocking solution for 30 minutes at room temperature before incubation with primary antibody (1/300) overnight at 4°C. Staining was detected with DAB.
Uckelmann H et al. Extracellular matrix protein Matrilin-4 regulates stress-induced HSC proliferation via CXCR4. J Exp Med213:1961-71 (2016).
Read more (PubMed: 27573814) »
Szalai E et al. Fibrillin-2, tenascin-C, matrilin-2, and matrilin-4 are strongly expressed in the epithelium of human granular and lattice type I corneal dystrophies. Mol Vis18:1927-36 (2012).
Read more (PubMed: 22876117) »