概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 40% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Predicted molecular weight: 18.2 kDa.
IHC-Fr 1/100 - 1/1000.
ELISA Use at an assay dependent concentration.

靶标

文献

This product has been referenced in:
  • Kanadia RN & Cepko CL Alternative splicing produces high levels of noncoding isoforms of bHLH transcription factors during development. Genes Dev 24:229-34 (2010). WB ; Mouse . Read more (PubMed: 20080942) »
  • Prasov L  et al. A critical analysis of Atoh7 (Math5) mRNA splicing in the developing mouse retina. PLoS One 5:e12315 (2010). IHC-Fr, WB ; Mouse . Read more (PubMed: 20808762) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Imaginal Discs of L3 larvae)
Specification
Imaginal Discs of L3 larvae
Username

Dr. Simon Weinberger

Verified customer

提交于 Sep 30 2013

I regret to inform you that the supplier which tested ab13536 is unable to provide me with an image for the staining of Math5. Please do not hesitate to contact me if you need further help or experience problems with the new protocol,

I am in touch with the laboratory to see if an image can be sourced, I appreciate very well that an image can help a lot to convince of the quality of the antibody and I am very keen on receiving one. I look forward to hearing how your next set of ...

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse embryonic head, including retina and brain)
Specification
Mouse embryonic head, including retina and brain
Fixative
Paraformaldehyde
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Dr. N Brown

Verified customer

提交于 Sep 11 2006

Thank you for your reply. I have received today the detailed protocol from the scientist who tested ab13536 and it confirmed my thoughts that the antibody is very sensitive to a particular protocol. As this information was not available to you at th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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