Anti-MATH1/HATH1抗体[Math1] (ab27667)

概述

  • 产品名称Anti-MATH1/HATH1抗体[Math1]
    参阅全部 MATH1/HATH1 一抗
  • 描述
    小鼠单克隆抗体[Math1] to MATH1/HATH1
  • 经测试应用适用于: IHC-P, IHC (PFA fixed), ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    不与反应: Chicken
  • 免疫原

    Recombinant fusion protein of Mouse MATH1

性能

应用

Our Abpromise guarantee covers the use of ab27667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC (PFA fixed) 1/1000. Mouse embryos were fixed in 4% PFA in phosphate buffer for 2 h at 4 degrees. Rinsed well with phosphate buffer including an overnight rinse (all at 4 degrees). Sunk in 30% sucrose/phos buffer and embedded in OCT for cryosection. Inconclusive results obtained by WB (please see abreview)
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATH1 antibody
    • ATOH1 antibody
    • ATOH1_HUMAN antibody
    • Atonal homolog 1 (Drosophila) antibody
    • Atonal homolog 1 antibody
    • bHLHa14 antibody
    • Class A basic helix-loop-helix protein 14 antibody
    • hATH1 antibody
    • Helix loop helix protein hATH 1 antibody
    • Helix-loop-helix protein hATH-1 antibody
    • MATH 1 antibody
    • MATH1 antibody
    • Protein atonal homolog 1 antibody
    see all

Anti-MATH1/HATH1 antibody [Math1] 图像

  • Mouse E10.5 neural tube stained with Math1 antibody ab27667 (red). Embryos were fixed in 4% PFA in phosphate buffer for 2 h at 4 degrees. Rinsed well with phosphate buffer including an overnight rinse (all at 4 degrees). Sunk in 30% sucrose/phos buffer and embedded in OCT for cryosection. Math1 antibody ab27667 was used at 1/1000 dilution.
  • ab27667 staining mouse adrenal tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking.  The primary antibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C.  A HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

Anti-MATH1/HATH1 antibody [Math1] (ab27667)参考文献

This product has been referenced in:
  • Terrinoni A  et al. Role of p63 and the Notch pathway in cochlea development and sensorineural deafness. Proc Natl Acad Sci U S A 110:7300-5 (2013). WB ; Mouse . Read more (PubMed: 23589895) »
  • Wang J  et al. Differential gene expression in normal esophagus and Barrett's esophagus. J Gastroenterol 44:897-911 (2009). IHC-P ; Human . Read more (PubMed: 19468668) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Peritoneal tumor)
Permeabilization Yes - 0.3% Triton x 100
Specification Peritoneal tumor
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 24 2016

The full length protein was used as an immunogen for ab27667, and the epitope has not been mapped, so we are not sure what region of the MATH1 protein that the antibody binds to.

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Human Cell (peritonei tumor)
Specification peritonei tumor
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 24 2014

Thank you for your reply.
We do not have any other reports that this antibody is not working and we are monitoring the quality of our products weekly.
A new vial of ab27667 would also be covered by the Abpromise, as would a vial of an alterna...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will provide us with vital information f...

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Thank you for contacting us. We have 2 antibodies ab75354 and ab85513 in catalogues that are anti MATH1/ HATH1. These products are however not tested in IHC with mouse tissue sections. IHC is an untested application so these antibodies a...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MEF, F9, JAR, IMR32)
Loading amount 20 µg
Specification MEF, F9, JAR, IMR32
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Oct 29 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (adrenal)
Specification adrenal
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jun 23 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adrenal)
Specification adrenal
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Jun 23 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"