Anti-MATH1/HATH1抗体(ab85513)

概述

  • 产品名称Anti-MATH1/HATH1抗体
    参阅全部 MATH1/HATH1 一抗
  • 描述
    兔多克隆抗体to MATH1/HATH1
  • 经测试应用适用于: IHC-FoFr, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Horse, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 288-337 (QLDALHFSTF EDSALTAMMA QKNLSPSLPG SILQPVQEEN SKTSPRSHRS) of Human MATH1/HATH1, NP_005163.

  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab85513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-MATH1/HATH1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling MATH1/HATH1 with ab85513.

  • Anti-MATH1/HATH1 antibody (ab85513) at 1 µg/ml (in 5% skim milk / PBS buffer) + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 12%
  • ab85513 staining MATH1/HATH1 in murine cochlea tissue by Immunohistochemistry.Tissue was fixed in 4% paraformaldehyde, blocked with 2.5% NGS/ 0.5% Triton X-100 for 1 hour and then incubated with ab85513 at a 1/500 dilution for 24 hours at 4°C. The Alexa-Fluor 633 conjugated secondary was used at a 1/500 dilution. Top image: SG spiral ganglion, OC organ of corti. Middle image: CHCs crista hair cells and the organ of corti. Bottom image: HC hair cells.

Anti-MATH1/HATH1 antibody (ab85513)参考文献

ab85513 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We currently offer three anti-Atoh1 primary antibodies in our catalog. Two of these are also polyclonal, ab75354 and ab85513. We currently do not offer these products in a biotinylated form.

Our cata...

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Thank you for contacting us. We have 2 antibodies ab75354 and ab85513 in catalogues that are anti MATH1/ HATH1. These products are however not tested in IHC with mouse tissue sections. IHC is an untested application so these antibodies a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"