概述

  • 产品名称Anti-MAT2B抗体
    参阅全部 MAT2B 一抗
  • 描述
    兔多克隆抗体to MAT2B
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (EFQQNNWHAV GCGFRRARPK FEQVNLLDSN AVHHIIHDFQ PHVIVHCAAE) of human MAT2B (NP_877725).

  • 阳性对照
    • Human fetal liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab86506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S-adenosylmethionine inhibition.
  • 组织特异性Widely expressed.
  • 通路Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
  • 序列相似性Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110064C04Rik antibody
    • 2410018D16Rik antibody
    • AI182287 antibody
    • AU022853 antibody
    • Beta regulatory subunit of methionine adenosyltransferase antibody
    • dTDP 4 keto 6 deoxy D glucose 4 reductase antibody
    • DTDP-4-keto-6-deoxy-D-glucose 4-reductase antibody
    • MAT II antibody
    • MAT II beta antibody
    • Mat2b antibody
    • MAT2B_HUMAN antibody
    • MATIIbeta antibody
    • Methionine adenosyltransferase 2 beta subunit antibody
    • Methionine adenosyltransferase 2 subunit beta antibody
    • Methionine adenosyltransferase II beta antibody
    • MGC12237 antibody
    • MSTP045 antibody
    • Nbla02999 antibody
    • OTTMUSP00000005600 antibody
    • Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase antibody
    • Putative protein product of Nbla02999 antibody
    • RP23-382C18.2 antibody
    • SDR23E1 antibody
    • Short chain dehydrogenase/reductase family 23E member 1 antibody
    • TGR antibody
    see all

Anti-MAT2B antibody 图像

  • Anti-MAT2B antibody (ab86506) at 1 µg/ml + human fetal liver lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • ICC/IF image of ab86506 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86506, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MAT2B antibody (ab86506)参考文献

ab86506 has not yet been referenced specifically in any publications.

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