Anti-MAT tag抗体[MAT1-87] (ab50292)

概述

  • 产品名称Anti-MAT tag抗体[MAT1-87]
  • 描述
    小鼠单克隆抗体[MAT1-87] to MAT tag
  • 特异性ab50292 recognizes the seven amino acid MAT epitope sequence within either N or C terminal MAT-tagged fusion proteins
  • 经测试应用适用于: ICC/IF, WB, IP, ELISAmore details
  • 免疫原

    A synthetic MAT peptide

    HNHRHKHGGGC

    conjugated to KLH.

  • 阳性对照
    • Cell extracts expressing a C-terminal MAT-tagged protein.
  • 常规说明


    Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified immunoglobulin
  • Primary antibody说明Monoclonal antibodies reacting specifically with the MAT tag may be useful in various immuno detection techniques to identify the expression of a MAT fusion protein or to aid in isolation and purification when MAT fusion proteins are produced in prokaryotic or eukaryotic host cells.
  • 克隆单克隆
  • 克隆编号MAT1-87
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 1 kDa.
IP Use a concentration of 5 - 10 µg/ml.

5 - 10 µg can immunoprecipitate a recombinant C-terminal MAT-tagged protein.

ELISA Use at an assay dependent concentration.

靶标

  • 相关性The MAT tag is a seven amino acid peptide tag that binds to transition metals such as Nickel and Cobalt. This tag, which consists of the sequence HNHRHKH, allows the purification of MAT fusion proteins using immobilized metal affinity chromatography (IMAC) such as the highly selective HIS-Select® Nickel Affinity Gel. The MAT tag sequence can be incorporated into both prokaryotic and eukaryotic expression vectors.

Anti-MAT tag antibody [MAT1-87] (ab50292)参考文献

ab50292 has not yet been referenced specifically in any publications.

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