Anti-Mast Cell Tryptase抗体[AA1] (ab81703)


  • 产品名称Anti-Mast Cell Tryptase抗体[AA1]
    参阅全部 Mast Cell Tryptase 一抗
  • 描述
    小鼠单克隆抗体[AA1] to Mast Cell Tryptase
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Cat, Dog, Human, Monkey
  • 免疫原

    Human mast cell tryptase purified from human lung tissues.

  • 阳性对照
    • Tonsil
  • 常规说明

    ab81703 is an excellent marker for mast cells, and does not bind to any other cell type in Immunohistology.



Our Abpromise guarantee covers the use of ab81703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 31 kDa. For 2 hours at room temperature.
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.


  • 相关性Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Tryptase is considered to be an important marker of mast cell activation as well as an important mediator of inflammation.
  • 细胞定位Secreted. Note: released from the secretory granules upon mast cell activation.
  • 数据库链接
  • 别名
    • alpha II antibody
    • Lung tryptase antibody
    • Mast cell alpha II tryptase antibody
    • Mast cell beta I tryptase antibody
    • Mast cell protease 7 antibody
    • Mast cell protease II antibody
    • MCP 7 antibody
    • Pituitary tryptase antibody
    • Skin tryptase antibody
    • TPS 1 antibody
    • TPS1 antibody
    • TPS2 antibody
    • TPSAB1 antibody
    • TPSAB1 protein antibody
    • TPSB1 antibody
    • Tryptase 1 antibody
    • Tryptase alpha 1 antibody
    • tryptase alpha I included antibody
    • Tryptase alpha II antibody
    • tryptase alpha II included antibody
    • tryptase alpha included antibody
    • tryptase alpha/beta 1 antibody
    • Tryptase beta 1 antibody
    • tryptase beta I included antibody
    • Tryptase I antibody
    • tryptase I included antibody
    • Tryptase III antibody
    • Tryptase skin antibody
    see all

Anti-Mast Cell Tryptase antibody [AA1] 图像

  • Formalin fixed, paraffin embedded human tonsil stained with anti Mast Cell Tryptase (the form of ab81703 containing BSA and azide), at a 1/2000 dilution. Peroxidase conjugate and AEC chromogen were used. Note staining of mast cells.

Anti-Mast Cell Tryptase antibody [AA1] (ab81703)参考文献

This product has been referenced in:
  • Walls AF  et al. Production and characterization of monoclonal antibodies specific for human mast cell tryptase. Clin Exp Allergy 20:581-9 (1990). WB . Read more (PubMed: 2253091) »

See 1 Publication for this product

Product Wall

Thank you for your phone call. For ab81703, we recommend coating the Mast Cell Tryptase using a concentration between 2-20ug/mL in coating buffer:

3.03 g Na2CO3
6.0 g NaHCO3
1000 ml distilled water
pH 9.6

I hope this ...

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Thank you very much for your interest in ab81703.
To our knowledge, ab81703 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab81703 now, test it in rat and submit feedback to us in the form of an Abre...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (Mast Cell Tryptase)
Specification Mast Cell Tryptase
Blocking step Neptune Block with Non-mammalian Blockers as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Type Sandwich (Capture)

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提交于 Jun 02 2010