Anti-Mast Cell Chymase抗体(ab111239)

概述

  • 产品名称Anti-Mast Cell Chymase抗体
    参阅全部 Mast Cell Chymase 一抗
  • 描述
    山羊多克隆抗体to Mast Cell Chymase
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Mast Cell Chymase aa 96-110 (internal sequence) (Cysteine residue).
    Sequence:

    C-QFRHPKYNTSTLHHD

  • 阳性对照
    • Human testis and tonsil tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab111239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
  • 组织特异性Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.
  • 序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Secreted. Cytoplasmic granule. Mast cell granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-chymase antibody
    • Chymase 1 antibody
    • Chymase 1 mast cell antibody
    • chymase 1 preproprotein transcript E antibody
    • chymase 1 preproprotein transcript I antibody
    • Chymase antibody
    • Chymase, heart antibody
    • Chymase, mast cell antibody
    • CMA1 antibody
    • CMA1_HUMAN antibody
    • CYH antibody
    • CYM antibody
    • EC 3.4.21.39 antibody
    • Mast cell chymase 1 antibody
    • Mast cell protease 3 antibody
    • Mast cell protease 5 antibody
    • Mast cell protease I antibody
    • Mast cell protease III antibody
    • Mcp-5 antibody
    • MCP3P antibody
    • Mcpt5 antibody
    • MCT1 antibody
    • MGC119890 antibody
    • MGC119891 antibody
    • MMCP-5 antibody
    see all

Anti-Mast Cell Chymase antibody 图像

  • ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
  • ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.

Anti-Mast Cell Chymase antibody (ab111239)参考文献

This product has been referenced in:
  • Judd LM  et al. Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice. Am J Physiol Gastrointest Liver Physiol 310:G13-25 (2016). Mouse . Read more (PubMed: 26514775) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (nasal mucosa)
Permeabilization No
Specification nasal mucosa
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Duc Dung Le

Verified customer

提交于 Jun 09 2016

Thank you for contacting us.

The immunogen sequence of antibody ab2377 is Purified human skin Chymase, ab111239, Human testis and tonsil tissue and ab54176 is Purified human skin chymase.

We unfortunately do not have any extra immu...

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Vielen Dank für Ihre Antwort.

Ich kann bestätigen, dass sich Ihr Protokoll gut anhört. Falls Sie allerding die Mastzellen rot färben möchten, würde ich nicht Eosin als Gegenfärbung nehmen.

Allgemei...

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Vielen Dank für Ihre Anfrage.

Die Abbildung von ab54176 wurde mit einer Gegenfärbung mit Eosin (alleine) gefärbt.

Während die Abbildung von ab111239 eine Gegenfärbung mit Hematoxlin zeigt.

Beide k&...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"