Anti-Mast Cell Chymase抗体(ab111239)
Key features and details
- Goat polyclonal to Mast Cell Chymase
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Mast Cell Chymase抗体
参阅全部 Mast Cell Chymase 一抗 -
描述
山羊多克隆抗体to Mast Cell Chymase -
宿主
Goat -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Mast Cell Chymase aa 50-150 (internal sequence) (Cysteine residue).
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阳性对照
- Human testis and tonsil tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111239于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 1.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. -
组织特异性
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions. -
序列相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
细胞定位
Secreted. Cytoplasmic granule. Mast cell granules. - Information by UniProt
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数据库链接
- Entrez Gene: 1215 Human
- Omim: 118938 Human
- SwissProt: P23946 Human
- Unigene: 135626 Human
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别名
- Alpha-chymase antibody
- Chymase 1 antibody
- Chymase 1 mast cell antibody
see all
图片
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ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
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ab111239, at 1.5 µg/ml, staining Mast Cell Chymase in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen were used to develop staining.
数据表及文件
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Datasheet download
文献 (7)
ab111239 被引用在 7 文献中.
- Yamanaka-Takaichi M et al. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. Int J Mol Sci 22:N/A (2021). PubMed: 33803422
- Kovacs M et al. The pathogenic role of c-Kit+ mast cells in the spinal motor neuron-vascular niche in ALS. Acta Neuropathol Commun 9:136 (2021). PubMed: 34389060
- Trias E et al. Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis. Glia 68:1165-1181 (2020). PubMed: 31859421
- Valero-Jiménez A et al. Transmembrane protease, serine 4 (TMPRSS4) is upregulated in IPF lungs and increases the fibrotic response in bleomycin-induced lung injury. PLoS One 13:e0192963 (2018). PubMed: 29529050
- Trias E et al. Mast cells and neutrophils mediate peripheral motor pathway degeneration in ALS. JCI Insight 3:N/A (2018). PubMed: 30282815
- Judd LM et al. Elevated IL-33 expression is associated with pediatric eosinophilic esophagitis, and exogenous IL-33 promotes eosinophilic esophagitis development in mice. Am J Physiol Gastrointest Liver Physiol 310:G13-25 (2016). Mouse . PubMed: 26514775
- Buzzelli JN et al. IL33 Is a Stomach Alarmin That Initiates a Skewed Th2 Response to Injury and Infection. Cell Mol Gastroenterol Hepatol 1:203-221.e3 (2015). PubMed: 28210674