概述

  • 产品名称Anti-MASPIN抗体
    参阅全部 MASPIN 一抗
  • 描述
    兔多克隆抗体to MASPIN
  • 特异性This antibody detects endogenous levels of total MASPIN protein.
  • 经测试应用适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthesized peptide derived from an internal region of human MASPIN

  • 阳性对照
    • Extracts from Hela cells

性能

应用

Our Abpromise guarantee covers the use of ab65136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

靶标

  • 功能Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • 组织特异性Normal mammary epithelial cells.
  • 序列相似性Belongs to the serpin family. Ov-serpin subfamily.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Maspin antibody
    • Ovalbumin antibody
    • Peptidase inhibitor 5 antibody
    • PI-5 antibody
    • PI5 antibody
    • protease inhibitor 5 (maspin) antibody
    • Protease inhibitor 5 antibody
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpin B5 antibody
    • serpin family B member 5 antibody
    • Serpin peptidase inhibitor antibody
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody
    • Serpinb5 antibody
    • SPB5_HUMAN antibody
    • Spi5 antibody
    • Spi7 antibody
    see all

Anti-MASPIN antibody 图像

  • All lanes : Anti-MASPIN antibody (ab65136) at 1/500 dilution

    Lane 1 : Extracts from Hela cells
    Lane 2 : Extracts from Hela cells, plus immunising peptide


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ICC/IF image of ab65136 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65136, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MASPIN antibody (ab65136)参考文献

This product has been referenced in:
  • Todd AG  et al. SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro. J Mol Biol 401:681-9 (2010). Read more (PubMed: 20620147) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab65136.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"