概述

  • 产品名称
  • 描述
    兔多克隆抗体to MASP1
  • 特异性
    ab72659 detects endogenous levels of fragment of activated MASP1 resulting from cleavage adjacent to Arg448.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from human MASP1.

  • 阳性对照
    • Extracts from A549 cells treated with etoposide (25uM, 24hours).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
ELISA 1/10000.

靶标

  • 相关性
    MASP is a component of the bactericidal ra-reactive factor rarf which specifically binds to RA and R2 polysaccharides expressed by certain enterobacteria. It triggers the activation of complement cascade by activating the C4 and C2 components. It activates the C4 component by cleaving the alpha-chain of C4. The alternative-splicing product from MASP1 gene is called MASP3. MASP1 is associated with smaller MBL oligomers whereas MASP3 is found on larger oligomers. The substrate of MASP3 is unknown.
  • 细胞定位
    Secreted
  • 数据库链接
  • 别名
    • Complement factor MASP-3 antibody
    • Complement factor MASP3 antibody
    • Complement-activating component of Ra-reactive factor antibody
    • CRARF antibody
    • Mannose binding lectin associated serine protease 1 antibody
    • Mannose binding protein associated serine protease antibody
    • Mannose-binding lectin-associated serine protease 1 antibody
    • Mannose-binding lectin-associated serine protease-1 antibody
    • MASP antibody
    • PRSS 5 antibody
    • Ra-reactive factor serine protease p100 antibody
    • RaRF antibody
    • Serine protease 5 antibody
    see all

Anti-MASP1 antibody 图像

  • All lanes : Anti-MASP1 antibody (ab72659) at 1/500 dilution

    Lane 1 : Extracts from A549 cells, treated with etoposide (25uM, 24hours)
    Lane 2 : Extracts from A549 cells, treated with etoposide (25uM, 24hours) with Cl-peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-MASP1 antibody (ab72659)参考文献

ab72659 has not yet been referenced specifically in any publications.

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