概述

  • 产品名称Anti-MARVELD2抗体
    参阅全部 MARVELD2 一抗
  • 描述
    兔多克隆抗体to MARVELD2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 90 and 140 of human MARVELD2 (NP_001033692.1).

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 - 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 64 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Tight junctions (TJ) prevent leakage of solutes through the paracellular pathway of epithelial cells. MARVELD2, or tricellulin (TRIC), is an integral membrane protein concentrated at the vertically oriented TJ strands of tricellular contacts. It plays a role in the formation of the epithelial barriers.
    • 细胞定位Cell membrane; Multi-pass membrane protein. Cell junction, tight junction. Note=Found at tricellular contacts.
    • 数据库链接
    • 别名
      • DFNB49 antibody
      • FLJ30532 antibody
      • MARVD2 antibody
      • MARVEL (membrane associating) domain containing 2 antibody
      • MARVEL domain containing 2 antibody
      • MARVEL domain containing protein 2 antibody
      • MARVELD 2 antibody
      • MARVELD2 antibody
      • MRVLDC2 antibody
      • Tric antibody
      • Tricellulin antibody
      see all

    Anti-MARVELD2 antibody 图像

    • All lanes : Anti-MARVELD2 antibody (ab80421) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg

      Developed using the ECL technique

      Predicted band size : 64 kDa
      Observed band size : 64 kDa


      Exposure time : 10 minutes
    • Detection of human MARVELD2 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80421 at 3 µg/mg lysate for IP (lane 1). For subsequent WB detection, 1 µg/ml of ab80421 was used. Lane 2 represents control IgG IP.
      Detection: chemiluminescence with an exposure time of 10 seconds.

    Anti-MARVELD2 antibody (ab80421)参考文献

    This product has been referenced in:
    • Masuda R  et al. Negative regulation of the tight junction protein tricellulin by snail-induced epithelial-mesenchymal transition in gastric carcinoma cells. Pathobiology 77:106-13 (2010). Read more (PubMed: 20332670) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"