概述

  • 产品名称Anti-MARVELD1抗体
    参阅全部 MARVELD1 一抗
  • 描述
    兔多克隆抗体to MARVELD1
  • 特异性Specific for MARVELD1. Does not bind to other MARVELD proteins.
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    16 amino acids synthetic peptide from the N terminal region of Human MARVELD1 (EAW49903).

  • 阳性对照
    • Mouse heart tissue lysate This antibody gave a positive result when used in the following methanol fixed cell lines: PC3

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 19 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Microtubule-associated protein that exhibits cell cycle-dependent localization and can inhibit cell proliferation and migration.
  • 组织特异性Widely expressed in normal tissues. Down-regulated in multiple primary tumors.
  • 序列相似性Contains 1 MARVEL domain.
  • 细胞定位Cell membrane. Cytoplasm > cytoskeleton. Nucleus. Observed in the nucleus and at the perinuclear region during interphase, but localizes at the mitotic spindle and midbody at metaphase. A significant fraction of MARVELD1 translocates to the plasma membrane during anaphase or upon microtubule depolymerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GB14 antibody
    • MALD1_HUMAN antibody
    • MARVD1 antibody
    • MARVEL (membrane-associating) domain containing 1 antibody
    • MARVEL domain containing protein 1 antibody
    • MARVEL domain-containing protein 1 antibody
    • MARVELD1 antibody
    • MRVLDC1 antibody
    • Putative MARVEL domain containing protein 1 antibody
    see all

Anti-MARVELD1 antibody 图像

  • ICC/IF image of ab91640 stained PC3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91640 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Lane 1 : Anti-MARVELD1 antibody (ab91640) at 1 µg/ml
    Lane 2 : Anti-MARVELD1 antibody (ab91640) at 2 µg/ml

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse heart lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 19 kDa

Anti-MARVELD1 antibody (ab91640)参考文献

This product has been referenced in:
  • Hu J  et al. MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1. PLoS One 8:e68291 (2013). WB ; Human . Read more (PubMed: 23826386) »

See 1 Publication for this product

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