Anti-MARK4抗体(ab5262)
Key features and details
- Goat polyclonal to MARK4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MARK4抗体
参阅全部 MARK4 一抗 -
描述
山羊多克隆抗体to MARK4 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide:
IKRKPPPGCSDSPGV
, corresponding to C terminal amino acids 674-688 of Human MARK4. -
阳性对照
- WB: Human cerebellum tissue, mouse brain tissue, and U251 cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa.
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 75 kDa. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942). -
组织特异性
Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945). -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
发展阶段
Expressed at all stages of the mitotic cell cycle. -
翻译后修饰
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532). -
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus. - Information by UniProt
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数据库链接
- Entrez Gene: 57787 Human
- Entrez Gene: 232944 Mouse
- Omim: 606495 Human
- SwissProt: Q96L34 Human
- SwissProt: Q8CIP4 Mouse
- Unigene: 34314 Human
- Unigene: 260504 Mouse
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别名
- FLJ90097 antibody
- KIAA1860 antibody
- MAP / microtubule affinity regulating kinase 4 antibody
see all
图片
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All lanes : Anti-MARK4 antibody (ab5262) at 0.3 µg/ml
Lane 1 : Human cerebellum tissue lysate (35µg protein in RIPA buffer)
Lane 2 : Human cerebellum tissue lysate (35µg protein in RIPA buffer) with Immunizing peptide
Lane 3 : Mouse brain tissue lysate (35µg protein in RIPA buffer)
Lane 4 : Mouse brain tissue lysate (35µg protein in RIPA buffer) with Immunizing peptide
Developed using the ECL technique.
Predicted band size: 75 kDa -
Anti-MARK4 antibody (ab5262) at 1 µg/ml + U251 cell lysate (35µg protein in RIPA buffer)
Developed using the ECL technique.
Predicted band size: 75 kDa
数据表及文件
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Datasheet download
文献 (5)
ab5262 被引用在 5 文献中.
- Li L et al. Long non-coding RNA DLGAP1-AS1 promotes the progression of gastric cancer via miR-515-5p/MARK4 axis. Braz J Med Biol Res 54:e10062 (2021). PubMed: 34037089
- Ortega MA et al. Chronic venous disease patients show increased IRS-4 expression in the great saphenous vein wall. J Int Med Res 49:3000605211041275 (2021). PubMed: 34590920
- Chen Y et al. Capn4 regulates migration and invasion of ovarian carcinoma cells via targeting osteopontin-mediated PI3K/AKT signaling pathway. Oncol Lett 17:564-570 (2019). PubMed: 30655802
- Wang L et al. Prazosin protects myocardial cells against anoxia-reoxygenation injury via the extracellular signal-regulated kinase signaling pathway. Mol Med Rep 17:2145-2152 (2018). PubMed: 29207167
- Magnani I et al. Differential signature of the centrosomal MARK4 isoforms in glioma. Anal Cell Pathol (Amst) 34:319-38 (2011). PubMed: 22156016