Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain.
Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
All lanes : Anti-MARK2 antibody (ab113284) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 5 µg Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 88 kDa
Exposure time : 3 minutes
Immunoprecipitation - MARK2 antibody (ab113284)
ab113284 at 1 µg/ml staining MARK2 in 293T whole cell lysate immunoprecipitated using ab113284 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: controle IgG.
Predicted band size : 88 kDa.
Exposure time: 30 seconds.