功能Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection.
组织特异性High levels of expression in heart, brain, skeletal muscle and pancreas, lower levels observed in lung, liver and kidney.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain. Contains 1 protein kinase domain. Contains 1 UBA domain.
细胞定位Cell membrane. Phosphorylated by PRKCZ in polarized epithelial cells, resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane.
1 mg whole cell lysate from 293T cells was immunoprecipitated using Lane 1: ab113267 at 6 µg/mg lysate Lane 2: a Rabbit anti-MARK2 antibody that recognizes a downstream epitope
Lane 3: a control IgG. 20% of immunoprecipitates was loaded on the gel. For WB of immunoprecipitated MARK2, ab113267 was used at 1 µg/ml. Detection:
Chemiluminescence with an exposure time of 30 seconds.
Anti-MARK2 antibody (ab113267)参考文献
has not yet been referenced specifically in any publications.