重组Anti-MARCKS (phospho S158)抗体[EP2113Y] (ab81295)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2113Y] to MARCKS (phospho S158)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-MARCKS (phospho S158)抗体[EP2113Y]
参阅全部 MARCKS 一抗 -
描述
兔单克隆抗体[EP2113Y] to MARCKS (phospho S158) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Hela cell lysates treated with TPA.
-
常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2113Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81295于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
靶标
-
功能
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. -
序列相似性
Belongs to the MARCKS family. -
翻译后修饰
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. -
细胞定位
Cytoplasm > cytoskeleton. Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 4082 Human
- Entrez Gene: 17118 Mouse
- Entrez Gene: 25603 Rat
- Omim: 177061 Human
- SwissProt: P29966 Human
- SwissProt: P26645 Mouse
- SwissProt: P30009 Rat
- Unigene: 519909 Human
see all -
别名
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
图片
-
All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (purified)
Lane 1 : Rat brain
Lane 2 : Mouse brain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/10000 dilution (purified)
Lane 1 : HEK293 cells treated with TPA
Lane 2 : HEK293 cells treated with TPA, and the PVDF membrane treated with lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295) at 1/2000 dilution (unpurified)
Lane 1 : Hela cell lysates, untreated.
Lane 2 : Hela cell lysates treated with TPA.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab81295 被引用在 9 文献中.
- Zhong C et al. Protein Kinase C-Mediated Hyperphosphorylation and Lateralization of Connexin 43 Are Involved in Autoimmune Myocarditis-Induced Prolongation of QRS Complex. Front Physiol 13:815301 (2022). PubMed: 35418879
- Wang CN et al. Targeting the phosphorylation site of myristoylated alanine-rich C kinase substrate alleviates symptoms in a murine model of steroid-resistant asthma. Br J Pharmacol 176:1122-1134 (2019). PubMed: 30706455
- Yang DC et al. Tackling MARCKS-PIP3 circuit attenuates fibroblast activation and fibrosis progression. FASEB J 33:14354-14369 (2019). PubMed: 31661644
- Rohrbach TD et al. MARCKS phosphorylation is modulated by a peptide mimetic of MARCKS effector domain leading to increased radiation sensitivity in lung cancer cell lines. Oncol Lett 13:1216-1222 (2017). WB ; Human . PubMed: 28454237
- Rohrbach TD et al. Targeting the effector domain of the myristoylated alanine rich C-kinase substrate enhances lung cancer radiation sensitivity. Int J Oncol 46:1079-88 (2015). PubMed: 25524703
- Jarboe JS et al. MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma. Clin Cancer Res 18:3030-41 (2012). WB . PubMed: 22619307
- Rumsby M et al. Human prostate cell lines from normal and tumourigenic epithelia differ in the pattern and control of choline lipid headgroups released into the medium on stimulation of protein kinase C. Br J Cancer 104:673-84 (2011). WB ; Human . PubMed: 21266973
- Chen X & Rotenberg SA PhosphoMARCKS drives motility of mouse melanoma cells. Cell Signal 22:1097-103 (2010). PubMed: 20211725
- Gambaryan S et al. Thrombin and collagen induce a feedback inhibitory signaling pathway in platelets involving dissociation of the catalytic subunit of protein kinase A from an NFkappaB-IkappaB complex. J Biol Chem 285:18352-63 (2010). PubMed: 20356841