Anti-MARCKS (phospho S152 + S156)抗体(ab32590)
Key features and details
- Rabbit polyclonal to MARCKS (phospho S152 + S156)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-MARCKS (phospho S152 + S156)抗体
参阅全部 MARCKS 一抗 -
描述
兔多克隆抗体to MARCKS (phospho S152 + S156) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to MARCKS (phospho S152 + S156).
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阳性对照
- Rat brain lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32590于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 31 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 31 kDa). |
靶标
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功能
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. -
序列相似性
Belongs to the MARCKS family. -
翻译后修饰
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. -
细胞定位
Cytoplasm > cytoskeleton. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 25603 Rat
- SwissProt: P30009 Rat
- Unigene: 9560 Rat
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别名
- 80 kDa protein antibody
- 80K L antibody
- 80K L protein antibody
see all
图片
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All lanes : Anti-MARCKS (phospho S152 + S156) antibody (ab32590) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with Ser152/156 phosphopeptide used as antigen
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted?
The corresponding dephosphopeptide did not block the immunolabeling (not shown).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab32590 被引用在 1 文献中.
- Kim JH et al. Intramyocardial Adipose-Derived Stem Cell Transplantation Increases Pericardial Fat with Recovery of Myocardial Function after Acute Myocardial Infarction. PLoS One 11:e0158067 (2016). Flow Cyt . PubMed: 27336402