Anti-MARCKS抗体(ab72459)

概述

  • 产品名称Anti-MARCKS抗体
    参阅全部 MARCKS 一抗
  • 描述
    兔多克隆抗体to MARCKS
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Cow, Human
    预测可用于: all Mammals
  • 免疫原

    Recombinant full length rat MARCKS expressed in and purified from E. coli.

  • 阳性对照
    • rat cerebral cortex lysate, Mixed neuron/glial cultures.

性能

应用

Our Abpromise guarantee covers the use of ab72459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 30 kDa).
ICC/IF 1/500.

靶标

  • 功能MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • 序列相似性Belongs to the MARCKS family.
  • 翻译后修饰Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • 细胞定位Cytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS antibody 图像

  • Anti-MARCKS antibody (ab72459) at 1/10000 dilution + rat cerebral cortex lysate

    Predicted band size : 30 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Mixed neuron/glial cultures stained with MARCKS antibody ab72459 at 1/500 (red), and with an anti-MAP2 antibody (green).

Anti-MARCKS antibody (ab72459)参考文献

ab72459 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Oligodendrocyte)
Loading amount 30 µg
Specification Oligodendrocyte
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 27°C
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提交于 May 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Oligodendrocyte)
Specification Oligodendrocyte
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 27°C
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Abcam user community

Verified customer

提交于 May 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"