概述

  • 产品名称
  • 描述
    兔多克隆抗体to MARCKS
  • 特异性
    ab51100 detects endogenous levels of total MARCKS protein.
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthentic non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-SP-F-K).

  • 阳性对照
    • Human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab51100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Incubate for 1 hour.
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

靶标

  • 功能
    MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • 序列相似性
    Belongs to the MARCKS family.
  • 翻译后修饰
    Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • 细胞定位
    Cytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

图片

  • ab51100 at 1/100 dilution staining MARCKS in paraffin embedded human brain sections by Immunohistochemistry, in the absence and presence of the immunizing (blocking) peptide.
  • Anti-MARCKS antibody (ab51100) at 1/2000 dilution + 293 cells, lysed in RIPA buffer containing minicomplete protease inhibitors at 30 µg

    Secondary
    HRP conjugated goat anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

This product has been referenced in:
  • Johnston DGW  et al. MicroRNA-21 Limits Uptake of Listeria monocytogenes by Macrophages to Reduce the Intracellular Niche and Control Infection. Front Cell Infect Microbiol 7:201 (2017). Read more (PubMed: 28589100) »
  • Qi X  et al. Cathepsin B modulates lysosomal biogenesis and host defense against Francisella novicida infection. J Exp Med 213:2081-97 (2016). WB . Read more (PubMed: 27551156) »

See all 3 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells)
Loading amount
30 µg
Specification
293 cells
Gel Running Conditions
Reduced Denaturing (12% neutral pH)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jun 09 2008

Thank you for contacting us.

These antibodies will bind to the total protein (phospho and non-phospho).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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