概述

  • 产品名称Anti-MARCKS抗体
    参阅全部 MARCKS 一抗
  • 描述
    兔多克隆抗体to MARCKS
  • 特异性ab51100 detects endogenous levels of total MARCKS protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthentic non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-SP-F-K).

  • 阳性对照
    • Human brain tissue

性能

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应用

Our Abpromise guarantee covers the use of ab51100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Incubate for 1 hour.
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

靶标

  • 功能MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
  • 序列相似性Belongs to the MARCKS family.
  • 翻译后修饰Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
  • 细胞定位Cytoplasm > cytoskeleton. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 80 kDa protein antibody
    • 80K L antibody
    • 80K L protein antibody
    • 80K-L protein antibody
    • 80KL antibody
    • 81 kDa protein, light chain antibody
    • light chain antibody
    • MACS antibody
    • MARCKS antibody
    • MARCS antibody
    • MARCS_HUMAN antibody
    • MGC52672 antibody
    • myristoylated alanine rich C kinase substrate antibody
    • Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody
    • Myristoylated alanine rich protein kinase C substrate antibody
    • Myristoylated alanine-rich C-kinase substrate antibody
    • Phosphomyristin antibody
    • PKCSL antibody
    • PRKCSL antibody
    • protein kinase C substrate 80 kDa protein light chain antibody
    • Protein kinase C substrate antibody
    see all

Anti-MARCKS antibody 图像

  • ab51100 at 1/100 dilution staining MARCKS in paraffin embedded human brain sections by Immunohistochemistry, in the absence and presence of the immunizing (blocking) peptide.
  • Anti-MARCKS antibody (ab51100) at 1/2000 dilution + 293 cells, lysed in RIPA buffer containing minicomplete protease inhibitors at 30 µg

    Secondary
    HRP conjugated goat anti-rabbit
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-MARCKS antibody (ab51100)参考文献

This product has been referenced in:
  • Rodriguez Peña MJ  et al. MARCKS Protein Is Phosphorylated and Regulates Calcium Mobilization during Human Acrosomal Exocytosis. PLoS One 8:e64551 (2013). WB ; Human . Read more (PubMed: 23704996) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Loading amount 30 µg
Specification 293 cells
Gel Running Conditions Reduced Denaturing (12% neutral pH)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

提交于 Jun 09 2008

Thank you for contacting us.

These antibodies will bind to the total protein (phospho and non-phospho).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"