The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 27 kDa).
功能E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.
通路Protein modification; protein ubiquitination.
序列相似性Contains 1 RING-CH-type zinc finger.
结构域The RING-CH-type zinc finger domain is required for E3 ligase activity.