The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. ab117821 can also be used for IHC-IF. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
功能May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
序列相似性Belongs to the MAPRE family. Contains 1 CH (calponin-homology) domain. Contains 1 EB1 C-terminal domain.
结构域Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Associated with the microtubule network at the growing distal tip of microtubules. Also enriched at the centrosome.
Detection of Human MAPRE1 by Immunohistochemistry. Sample:
Formalin Fixed Paraffin Embedded section of Human prostate carcinoma. Antibody: ab117821 used at a dilution
of 1/100. Detection: Red-fluorescent Goat anti-Rabbit IgG heavy and light chain cross-adsorbed Antibody DyLight 594
Conjugated used at a dilution of 1/100.