Anti-MAPKAP Kinase 3抗体(ab64972)


  • 产品名称Anti-MAPKAP Kinase 3抗体
    参阅全部 MAPKAP Kinase 3 一抗
  • 描述
    兔多克隆抗体to MAPKAP Kinase 3
  • 经测试应用适用于: IHC-P, ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from internal region of human MAPKAP Kinase 3.

  • 阳性对照
    • Human skeletal muscle tissue. Extracts from COLO205 cells.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab64972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.


  • 功能Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
  • 组织特异性Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313.
  • 细胞定位Nucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3pK antibody
    • anti MAPKAPK3 antibody
    • Chromosome 3p kinase antibody
    • EC antibody
    • MAP kinase-activated protein kinase 3 antibody
    • MAPK activated protein kinase 3 antibody
    • MAPK-activated protein kinase 3 antibody
    • MAPK3_HUMAN antibody
    • MAPKAP antibody
    • MAPKAP kinase 3 antibody
    • MAPKAP-K3 antibody
    • MAPKAP3 antibody
    • MAPKAPK 3 antibody
    • MAPKAPK-3 antibody
    • Mapkapk3 antibody
    • MAPKAPK3 antibody antibody
    • Mitogen activated protein kinase activated protein kinase 3 antibody
    • Mitogen activated protein kinase activated protein kinase antibody
    • MK-3 antibody
    see all

Anti-MAPKAP Kinase 3 antibody 图像

  • All lanes : Anti-MAPKAP Kinase 3 antibody (ab64972) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells, without immunising peptide
    Lane 2 : extracts from COLO205 cells, with immunising peptide

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using MAPKAP Kinase 3 antibody (ab64972) at 1/50 dilution. Left: no immunising peptide. Right: with immunising peptide.
  • ICC/IF image of ab64972 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64972, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MAPKAP Kinase 3 antibody (ab64972)参考文献

ab64972 has not yet been referenced specifically in any publications.

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