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RabMAb

Anti-MAPKAP Kinase 2抗体[E341] (ab32567)

概述

  • 产品名称
    Anti-MAPKAP Kinase 2抗体[E341]
    参阅全部 MAPKAP Kinase 2 一抗
  • 描述
    兔单克隆抗体[E341] to MAPKAP Kinase 2
  • 特异性
    ab32567 recognises MAPKAP Kinase-2.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2.

  • 阳性对照
    • Hela cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab32567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
IP 1/200.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC.
  • 靶标

    • 功能
      Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
    • 组织特异性
      Expressed in all tissues examined.
    • 序列相似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Phosphorylated and activated by MAP kinase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MAP kinase activated protein Kinase 2 antibody
      • MAP kinase-activated protein kinase 2 antibody
      • MAPK activated protein kinase 2 antibody
      • MAPK-activated protein kinase 2 antibody
      • MAPK2_HUMAN antibody
      • MAPKAP K2 antibody
      • MAPKAP kinase 2 antibody
      • MAPKAPK 2 antibody
      • MAPKAPK-2 antibody
      • MAPKAPK2 antibody
      • Mitogen activated protein kinase activated protein kinase 2 antibody
      • MK 2 antibody
      • MK2 antibody
      see all

    图片



    • Predicted band size : 46 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: A431 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/1000 dilution + Hela cell lysate

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Lanes 1 - 2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
      Lanes 3 - 4 : Anti-MAPKAP Kinase 2 antibody [E341] (ab32567) at 1/500 dilution

      Lane 1 : UV treated HeLa cells
      Lane 2 : UV treated HeLa cells + p38 inhibitor
      Lane 3 : UV treated HeLa cells
      Lane 4 : UV treated HeLa cells + p38 inhibitor

      Secondary
      IRDye® 680 LICOR goat anti-rabbit.

      Performed under reducing conditions.

      Predicted band size : 46 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cross reactivity).

      Exposure time : 1 minuteab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).

      See Abreview

    文献

    ab32567 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    35 µg
    Specification
    HeLa cells
    Treatment
    UV treated samples (20 J dose)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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