重组Anti-MK2抗体[E341] (ab32567)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E341] to MK2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MK2抗体[E341]
参阅全部 MK2 一抗 -
描述
兔单克隆抗体[E341] to MK2 -
宿主
Rabbit -
特异性
ab32567 recognises MAPKAP Kinase-2. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human MK2 aa 350-450 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E341 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32567于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). |
靶标
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功能
Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. -
组织特异性
Expressed in all tissues examined. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated and activated by MAP kinase. - Information by UniProt
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数据库链接
- Entrez Gene: 9261 Human
- Entrez Gene: 17164 Mouse
- Entrez Gene: 289014 Rat
- Omim: 602006 Human
- SwissProt: P49137 Human
- SwissProt: P49138 Mouse
- Unigene: 643566 Human
- Unigene: 713747 Human
see all -
别名
- MAP kinase activated protein Kinase 2 antibody
- MAP kinase-activated protein kinase 2 antibody
- MAPK activated protein kinase 2 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MAPKAP Kinase 2knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32567 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32567 was shown to specifically react with MAPKAP Kinase 2 when MAPKAP Kinase 2 knockout samples were used. Wild-type and MAPKAP Kinase 2 knockout samples were subjected to SDS-PAGE. ab32567 and ab8245 (loading control to ProteinX2) were diluted to 1/500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Anti-MK2 antibody [E341] (ab32567) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 46 kDa
Observed band size: 46 kDa -
Lanes 1-2 : Anti-MK2 (phospho T334) antibody [EPR992Y] (ab51018) at 1/500 dilution
Lanes 3-4 : Anti-MK2 antibody [E341] (ab32567) at 1/500 dilution
Lanes 1 & 3 : UV treated HeLa cells
Lanes 2 & 4 : UV treated HeLa cells + p38 inhibitor
Secondary
All lanes : IRDye® 680 LICOR goat anti-rabbit.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cross reactivity)
Exposure time: 1 minute
ab32567 recognise total MK2 (lane 3,4). A control for specificity is shown as UV treated HeLa + p38 inhibitor (lane 2,4). While the intensity of pT334 band is greatly reduced (lane 2), total MK2 remains unaltered (lane 4).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab32567 被引用在 2 文献中.
- Muraleva NA et al. SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32731533
- McCaskill JL et al. Broad-Spectrum Inhibition of Respiratory Virus Infection by MicroRNA Mimics Targeting p38 MAPK Signaling. Mol Ther Nucleic Acids 7:256-266 (2017). Dog, Human . PubMed: 28624201