Anti-MAPKAP Kinase 2抗体(ab63574)


  • 产品名称Anti-MAPKAP Kinase 2抗体
    参阅全部 MAPKAP Kinase 2 一抗
  • 描述
    兔多克隆抗体to MAPKAP Kinase 2
  • 特异性ab63574 detects endogenous levels of total MAPKAP Kinase 2 protein. The immunogen sequence used to produce ab63574 is 77% identical to the corresponding region of MAPKAP Kinase 3. Customer correspondance suggests ab63574 may cross react with human MAPKAP Kinase 3 but this has not been confirmed.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human MAPKAP Kinase 2 . Synthetic non phosphopeptide derived from human MAPKAP Kinase 2 around the phosphorylation site of threonine 334 (P-Q-TP-P-L).

  • 阳性对照
    • IHC-P: Human breast carcinoma tissue WB: HeLa cell extracts treated with Ca2+ (40nM, 30mins).



Our Abpromise guarantee covers the use of ab63574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.


  • 功能Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
  • 组织特异性Expressed in all tissues examined.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated and activated by MAP kinase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAP kinase activated protein Kinase 2 antibody
    • MAP kinase-activated protein kinase 2 antibody
    • MAPK activated protein kinase 2 antibody
    • MAPK-activated protein kinase 2 antibody
    • MAPK2_HUMAN antibody
    • MAPKAP K2 antibody
    • MAPKAP kinase 2 antibody
    • MAPKAPK 2 antibody
    • MAPKAPK-2 antibody
    • MAPKAPK2 antibody
    • Mitogen activated protein kinase activated protein kinase 2 antibody
    • MK 2 antibody
    • MK2 antibody
    see all

Anti-MAPKAP Kinase 2 antibody 图像

  • ab63574, at 1/50 dilution, staining MAPKAP Kinase 2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
  • All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated with Ca2+ (40nM, 30mins)
    Lane 2 : HeLa cell extracts treated with Ca2+ (40nM, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-MAPKAP Kinase 2 antibody (ab63574) at 1/500 dilution

    Lane 1 : Recombinant Human MAPKAP Kinase 2 protein (ab79910) at 0.01 µg
    Lane 2 : Recombinant Human MAPKAP Kinase 2 protein (ab79910) at 0.001 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

Anti-MAPKAP Kinase 2 antibody (ab63574)参考文献

ab63574 has not yet been referenced specifically in any publications.

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