概述

  • 产品名称Anti-MAPK6抗体
    参阅全部 MAPK6 一抗
  • 描述
    兔多克隆抗体to MAPK6
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide designed within residues: NKQLESIGIP QFHSPVGSPL KSIQATLTPS AMKSSPQIPH QTYSSILKHL N, corresponding to amino acids 671-721 of Human MAPK6 (NP_002739.1)

  • 阳性对照
    • Hela, 293T or NIH 3T3 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab95079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 83 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
  • 组织特异性Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK-3 antibody
    • ERK3 antibody
    • Extracellular signal regulated kinase 3 antibody
    • Extracellular signal regulated kinase p97 antibody
    • Extracellular signal-regulated kinase 3 antibody
    • HsT17250 antibody
    • MAP kinase 6 antibody
    • MAP kinase isoform p97 antibody
    • MAPK 6 antibody
    • MAPK6 antibody
    • Mitogen activated protein kinase 6 antibody
    • Mitogen-activated protein kinase 6 antibody
    • MK06_HUMAN antibody
    • p97 MAPK antibody
    • p97-MAPK antibody
    • PRKM6 antibody
    • Protein kinase mitogen activated 5 antibody
    • Protein kinase mitogen activated 6 antibody
    see all

Anti-MAPK6 antibody 图像

  • Lane 1: 1mg HeLa whole cell lysate was immunoprecipitated with 3µg ab95079. 20% IP was loaded on gel and probed with 1µg/ml ab95079.
    Lane 2: same, using a control IgG
    Detection: chemiluminescence with exposure time of 30 seconds.
  • All lanes : Anti-MAPK6 antibody (ab95079) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH 3T3 cell lysate at 50 µg


    Predicted band size : 83 kDa

Anti-MAPK6 antibody (ab95079)参考文献

ab95079 has not yet been referenced specifically in any publications.

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