概述

  • 产品名称Anti-MAPK4抗体
    参阅全部 MAPK4 一抗
  • 描述
    兔多克隆抗体to MAPK4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide from within internal sequence amino acids 288-337: FLEKILTFNP MDRLTAEMGL QHPYMSPYSC PEDEPTSQHP FRIEDEIDDI, of Human MAPK4 (NP_002738)

  • 阳性对照
    • Human fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab94617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.
  • 组织特异性High expression in heart and brain.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The FRIEDE motif is required for docking MAPKAPK5.
    In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Phosphorylated at Ser-186 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK-4 antibody
    • ERK3 antibody
    • Erk3 related antibody
    • ERK4 antibody
    • Extracellular signal regulated kinase 4 antibody
    • Extracellular signal-regulated kinase 4 antibody
    • MAP kinase 4 antibody
    • MAP kinase isoform p63 antibody
    • MAPK 4 antibody
    • MAPK4 antibody
    • Mitogen activated protein kinase 4 antibody
    • Mitogen-activated protein kinase 4 antibody
    • MK04_HUMAN antibody
    • p63 MAPK antibody
    • p63-MAPK antibody
    • p63MAPK antibody
    • PRKM4 antibody
    • Protein kinase, mitogen-activated, 4 antibody
    • Protein-tyrosine kinase ERK3-related antibody
    • RMNK2 antibody
    see all

Anti-MAPK4 antibody 图像

  • Anti-MAPK4 antibody (ab94617) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa

Anti-MAPK4 antibody (ab94617)参考文献

ab94617 has not yet been referenced specifically in any publications.

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