Anti-MAPK15 (phospho T175 + Y177)抗体(ab73209)


  • 产品名称Anti-MAPK15 (phospho T175 + Y177)抗体
    参阅全部 MAPK15 一抗
  • 描述
    兔多克隆抗体to MAPK15 (phospho T175 + Y177)
  • 特异性ab73209 detects endogenous levels of MAPK15 only when phosphorylated at threonine 175 and tyrosine 177 (Human) or threonine 176 and tyrosine 178 (Mouse).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human MAPK15 around the phosphorylation site of threonine 175 and tyrosine 177 (AVTPEYPVA)

  • 阳性对照
    • Jurkat cell extract.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab73209 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab73209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).
ELISA 1/5000.


  • 功能In vitro, phosphorylates MBP.
  • 组织特异性Widely expressed with a maximal expression in lung and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK-7 antibody
    • ERK-8 antibody
    • ERK7 antibody
    • ERK8 antibody
    • Extracellular regulated kinase 8 delta antibody
    • Extracellular signal regulated kinase 8 antibody
    • Extracellular signal-regulated kinase 7 antibody
    • Extracellular signal-regulated kinase 8 antibody
    • MAP kinase 15 antibody
    • MAPK 15 antibody
    • Mapk15 antibody
    • Mitogen activated protein kinase 15 antibody
    • Mitogen-activated protein kinase 15 antibody
    • MK15_HUMAN antibody
    see all

Anti-MAPK15 (phospho T175 + Y177) antibody 图像

  • All lanes : Anti-MAPK15 (phospho T175 + Y177) antibody (ab73209) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 60 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-MAPK15 (phospho T175 + Y177) antibody (ab73209)参考文献

ab73209 has not yet been referenced specifically in any publications.

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