Anti-ERK8 (phospho T175 + Y177)抗体(ab73209)
Key features and details
- Rabbit polyclonal to ERK8 (phospho T175 + Y177)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ERK8 (phospho T175 + Y177)抗体
参阅全部 ERK8 一抗 -
描述
兔多克隆抗体to ERK8 (phospho T175 + Y177) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human ERK8 (phospho T175 + Y177).
Sequence:AVTPEYPVA
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阳性对照
- Jurkat cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab73209 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73209于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 60 kDa). |
靶标
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功能
In vitro, phosphorylates MBP. -
组织特异性
Widely expressed with a maximal expression in lung and kidney. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. - Information by UniProt
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数据库链接
- Entrez Gene: 225689 Human
- Entrez Gene: 332110 Mouse
- SwissProt: Q8TD08 Human
- SwissProt: Q80Y86 Mouse
- Unigene: 493169 Human
- Unigene: 40843 Mouse
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别名
- ERK 7 antibody
- ERK 8 antibody
- ERK-7 antibody
see all
图片
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All lanes : Anti-ERK8 (phospho T175 + Y177) antibody (ab73209) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab73209 被引用在 1 文献中.
- Jin DH et al. Overexpression of MAPK15 in gastric cancer is associated with copy number gain and contributes to the stability of c-Jun. Oncotarget 6:20190-203 (2015). WB . PubMed: 26035356