Anti-MAPK15抗体- N-terminal (ab70911)


  • 产品名称Anti-MAPK15抗体- N-terminal
    参阅全部 MAPK15 一抗
  • 描述
    兔多克隆抗体to MAPK15 - N-terminal
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the N-terminal region of human MAPK15

  • 阳性对照
    • T47D cell lysate; Paraffin embedded Human breast carcinoma tissue


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab70911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 60 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.


  • 功能In vitro, phosphorylates MBP.
  • 组织特异性Widely expressed with a maximal expression in lung and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK-7 antibody
    • ERK-8 antibody
    • ERK7 antibody
    • ERK8 antibody
    • Extracellular regulated kinase 8 delta antibody
    • Extracellular signal regulated kinase 8 antibody
    • Extracellular signal-regulated kinase 7 antibody
    • Extracellular signal-regulated kinase 8 antibody
    • MAP kinase 15 antibody
    • MAPK 15 antibody
    • Mapk15 antibody
    • Mitogen activated protein kinase 15 antibody
    • Mitogen-activated protein kinase 15 antibody
    • MK15_HUMAN antibody
    see all

Anti-MAPK15 antibody - N-terminal 图像

  • All lanes : Anti-MAPK15 antibody - N-terminal (ab70911) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell line lysate
    Lane 2 : 293 cell line lysate transiently transfected with the MAPK15 gene

    Lysates/proteins at 2 µg per lane.

    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Anti-MAPK15 antibody - N-terminal (ab70911) at 1/60 dilution + T47D cell line lysates at 35 µg

    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab70911, at a 1/10 dilution, staining human MAPK15 in breast carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70911 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Anti-MAPK15 antibody - N-terminal (ab70911)参考文献

ab70911 has not yet been referenced specifically in any publications.

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