概述

  • 产品名称Anti-MAPK15抗体
    参阅全部 MAPK15 一抗
  • 描述
    兔多克隆抗体to MAPK15
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 395-444 (HDPAEHESPRAAKNVPRQNSAPLLQTALLGNGERPPGAKEAPPLTLSLV K) of Human MAPK15 (NP_620590).

  • 阳性对照
    • MCF7 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能In vitro, phosphorylates MBP.
  • 组织特异性Widely expressed with a maximal expression in lung and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteasomal degradation.
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.
    Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ERK-7 antibody
    • ERK-8 antibody
    • ERK7 antibody
    • ERK8 antibody
    • Extracellular regulated kinase 8 delta antibody
    • Extracellular signal regulated kinase 8 antibody
    • Extracellular signal-regulated kinase 7 antibody
    • Extracellular signal-regulated kinase 8 antibody
    • MAP kinase 15 antibody
    • MAPK 15 antibody
    • Mapk15 antibody
    • Mitogen activated protein kinase 15 antibody
    • Mitogen-activated protein kinase 15 antibody
    • MK15_HUMAN antibody
    see all

Anti-MAPK15 antibody 图像



  • Predicted band size : 60 kDa
    Western blot analysis of untransfected HEK-293T (lane 1) and MAPK15 transfected HEK-293T (lane 2) lysates (50µg/lane) labeling MAPK15 with ab86275 at 1:2000. A HRP-conjugtaed anti-rabbit (1:1000) was used as the secondary antibody.
  • Sample Type : HA-MAPK15 transfected HeLa cells. Primary Antibody Dilution : 1:200. Secondary Antibody : Anti-rabbit-Alexa Fluor 555. Secondary Antibody Dilution : 1:800. Color/Signal Descriptions : A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: Merge. Gene Name : MAPK15.
  • Anti-MAPK15 antibody (ab86275) at 1 µg/ml (5% skim milk/ PBS buffer) + MCF7 cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

Anti-MAPK15 antibody (ab86275)参考文献

ab86275 has not yet been referenced specifically in any publications.

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