Anti-MAP4K6抗体(ab86385)

概述

  • 产品名称Anti-MAP4K6抗体
    参阅全部 MAP4K6 一抗
  • 描述
    兔多克隆抗体to MAP4K6
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 775 and 825 of Human MAP4K6 (NP_722549.2).

  • 阳性对照
    • Whole cell lysates from HeLa and mouse NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab86385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 150 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性MAP4K6 is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. These kinases regulate a wide variety of cellular processes by coupling cell surface receptors to MAPK pathways. This kinase appears to act upstream of the JUN N-terminal pathway and may be involved in brain development. It may also play a role in the response to environmental stress, in cytoskeleton reorganization, cell adhesion, and cell motility. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.
  • 细胞定位Cytoplasm. Cell junction; synapse; postsynaptic cell membrane; postsynaptic density. Cell projection; axon. Cell projection; dendrite. Isoform 4: Golgi apparatus
  • 数据库链接
  • 别名
    • B55 antibody
    • GCK family kinase MiNK antibody
    • hMINK antibody
    • Map4k6 antibody
    • MAPK/ERK kinase kinase kinase 6 antibody
    • MEK (MAP/ERK) kinase kinase 6 antibody
    • MEK kinase kinase 6 antibody
    • MEKKK6 antibody
    • MGC21111 antibody
    • MINK 1 antibody
    • MINK antibody
    • MINK1 antibody
    • Misshapen like kinase 1 antibody
    • Misshapen NIKs Related kinase antibody
    • Misshapen/NIK related kinase antibody
    • Mitogen activated protein kinase kinase kinase kinase 6 antibody
    • RP23-122P1.6 antibody
    • Ysk2 antibody
    • ZC3 antibody
    see all

Anti-MAP4K6 antibody 图像

  • All lanes : Anti-MAP4K6 antibody (ab86385) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 150 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Detection of MAP4K6 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86385 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ICC/IF image of ab86385 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86385, 5µg/ml) overnight at +4°C. The secondary antibody (green) was goat anti-rabbit DyLight 488 (IgG H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MAP4K6 antibody (ab86385)参考文献

ab86385 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% separating gel)
Sample Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification Hippocampal homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Feb 17 2014

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