Synthetic peptide, corresponding to a region within amino acids 417-482 (HCPDSESRAP QILRRQSSPS CGPVAETSSI GNGDGISKLM SENTEGSAQA PQLPRKNDKR DFPKPA) of Human MAP4K5 (NP_942089).
MOLT4 whole cell lysate; A431 cells; SAS xenograft; 293T, H1299, HepG2 and Raji cell lines
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 95 kDa.
1/100 - 1/500.
1/100 - 1/200.
MAP4K5, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress. It appears to act upstream of the JUN N-terminal pathway. MAP4K5 interacts with both SH3 domains of the adapter proteins CRK and CRKL. This cytoplasmic protein is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate.
EC 18.104.22.168 antibody
Kinase homologous to SPS1/STE20 antibody
Western blot - MAP4K5 antibody (ab96551)
Anti-MAP4K5 antibody (ab96551) at 1/5000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 95 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - MAP4K5 antibody (ab96551)
ab96551, at 1/200 dilution, staining MAP4K5 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP4K5 antibody (ab96551)
ab96551, at 1/100 dilution, staining MAP4K5 in paraffin-embedded SAS xenograft by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"