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Our Abpromise guarantee covers the use of ab56569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
|Flow Cyt||Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MAP4K4 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab56569 observed at 130 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab56569 was shown to recognize MAP4K4 when MAP4K4 knockout samples were used, along with additional cross-reactive bands. Wild-type and MAP4K4 knockout samples were subjected to SDS-PAGE. Ab56569 and ab181602 (loading control to GAPDH) were diluted at 1 ug/ml and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ab56569 has not yet been referenced specifically in any publications.