概述

  • 产品名称Anti-MAP4K1抗体[EP630Y]
    参阅全部 MAP4K1 一抗
  • 描述
    兔单克隆抗体[EP630Y] to MAP4K1
  • 特异性ab33910 recognises hematopoietic progenitor kinase 1 (HPK1).
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: ICC or ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MAP4K1 aa 1-100 (N terminal).

  • 阳性对照
    • WB: Raji and Daudi (human Burkitt's lymphoma) whole cell lysates. IHC-P: Human liver tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab33910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Predicted molecular weight: 97 kDa.
IHC-P 1/50.
  • 应用说明Is unsuitable for ICC or ICC/IF.
  • 靶标

    • 功能May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.
    • 组织特异性Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CNH domain.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Hematopoietic progenitor kinase 1 antibody
      • Hematopoietic progenitor kinase antibody
      • HPK1 antibody
      • Human Hematopoietic Progenitor Kinase antibody
      • M4K1_HUMAN antibody
      • Map4k1 antibody
      • MAPK/ERK kinase kinase kinase 1 antibody
      • MEK kinase kinase 1 antibody
      • MEKKK 1 antibody
      • Mitogen activated protein kinase kinase kinase kinase 1 antibody
      • Mitogen-activated protein kinase kinase kinase kinase 1 antibody
      see all

    Anti-MAP4K1 antibody [EP630Y] 图像

    • All lanes : Anti-MAP4K1 antibody [EP630Y] (ab33910) at 1/5000 dilution

      Lane 1 : Daudi (human Burkitt's lymphoma) whole cell lysate
      Lane 2 : Raji (human Burkitt's lymphoma) whole cell lysate

      Lysates/proteins at 1/20 dilution per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

      Predicted band size : 97 kDa

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • ab33910 staining MAP4K1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

      Negative control 1: PBS in place of primary antibody.

    Anti-MAP4K1 antibody [EP630Y] (ab33910)参考文献

    This product has been referenced in:
    • Palmer RD  et al. Pediatric malignant germ cell tumors show characteristic transcriptome profiles. Cancer Res 68:4239-47 (2008). IHC-P ; Human . Read more (PubMed: 18519683) »

    See 1 Publication for this product

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