概述

  • 产品名称Anti-MAP3K9抗体- C-terminal
    参阅全部 MAP3K9 一抗
  • 描述
    兔多克隆抗体to MAP3K9 - C-terminal
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 阳性对照
    • Human breast carcinoma tissue and rat testis tissue and A375 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).

靶标

  • 功能Activates the JUN N-terminal pathway.
  • 组织特异性Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M3K9_HUMAN antibody
    • Map3k9 antibody
    • MEKK9 antibody
    • Mitogen activated protein kinase kinase kinase 9 antibody
    • Mitogen-activated protein kinase kinase kinase 9 antibody
    • Mixed lineage kinase 1 (tyr and ser/thr specificity) antibody
    • Mixed lineage kinase 1 antibody
    • MLK1 antibody
    • PRKE1 antibody
    see all

Anti-MAP3K9 antibody - C-terminal 图像

  • All lanes : Anti-MAP3K9 antibody - C-terminal (ab71628) at 1/100 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : rat testis tissue lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 118 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
  • ab71628 at 1/50 dilution, staining MAP3K9 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-MAP3K9 antibody - C-terminal (ab71628)参考文献

This product has been referenced in:
  • Tivnan A  et al. MicroRNA-34a is a potent tumor suppressor molecule in vivo in neuroblastoma. BMC Cancer 11:33 (2011). WB ; Human . Read more (PubMed: 21266077) »

See 1 Publication for this product

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