兔多克隆抗体to MAP3K9 - C-terminal
Mouse, Rat, Human
Human breast carcinoma tissue and rat testis tissue and A375 cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71628 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).
Activates the JUN N-terminal pathway.
Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. Contains 1 SH3 domain.
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site.
Information by UniProt
Western blot - MAP3K9 antibody - C-terminal (ab71628)
All lanes :
Anti-MAP3K9 antibody - C-terminal (ab71628) at 1/100 dilution
Lane 1 :
A375 cell lysate
Lane 2 :
rat testis tissue lysate
Lysates/proteins at 12.5 µg per lane.
Predicted band size:
Observed band size:
100 kDa (
why is the actual band size different from the predicted?
Additional bands at:
35 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MAP3K9 antibody - C-terminal (ab71628)
ab71628 at 1/50 dilution, staining MAP3K9 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"