概述

  • 产品名称
    Anti-MAP3K8 (phospho T290)抗体
    参阅全部 MAP3K8 一抗
  • 描述
    兔多克隆抗体to MAP3K8 (phospho T290)
  • 特异性
    This antibody detects endogenous levels of MAP3K8 only when phosphorylated at threonine 290.
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized phosphopeptide derived from human MAP3K8 around the phosphorylation site of threonine 290 (R-G-TP-E-I).

  • 阳性对照
    • Human brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab51214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB Use at an assay dependent concentration. PubMed: 21267413

靶标

  • 功能
    Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • 组织特异性
    Expressed in several normal tissues and human tumor-derived cell lines.
  • 序列相似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 发展阶段
    Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • 翻译后修饰
    Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AURA2 antibody
    • c COT antibody
    • Cancer Osaka thyroid oncogene antibody
    • CCOT antibody
    • COT antibody
    • COT proto oncogene serine/threonine protein kinase antibody
    • EST antibody
    • ESTF antibody
    • Ewing sarcoma transformant antibody
    • FLJ10486 antibody
    • M3K8_HUMAN antibody
    • MAP3K 8 antibody
    • MAP3K8 antibody
    • MEKK8 antibody
    • Mitogen activated protein kinase kinase kinase 8 antibody
    • Mitogen-activated protein kinase kinase kinase 8 antibody
    • Proto oncogene cCot antibody
    • Proto-oncogene c-Cot antibody
    • Serine/threonine protein kinase cot antibody
    • Serine/threonine-protein kinase cot antibody
    • TPL 2 antibody
    • TPL-2 antibody
    • TPL2 antibody
    • Tumor progression locus 2 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K8 (phospho-Thr290) at a 1/50 dilution.

    Left image: Un-treated

    Right image: Treated with phosphopeptide

文献

This product has been referenced in:
  • Jeong JH  et al. TPL2/COT/MAP3K8 (TPL2) Activation Promotes Androgen Depletion-Independent (ADI) Prostate Cancer Growth. PLoS One 6:e16205 (2011). WB ; Human . Read more (PubMed: 21267413) »

See 1 Publication for this product

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