Anti-MAP3K8 (phospho S400)抗体(ab111367)

概述

  • 产品名称Anti-MAP3K8 (phospho S400)抗体
    参阅全部 MAP3K8 一抗
  • 描述
    兔多克隆抗体to MAP3K8 (phospho S400)
  • 特异性ab111367 detects endogenous levels of MAP3K8 only when phosphorylated at serine 400.
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human MAP3K8 around the phosphorylation site of serine 400 (C-Q-SP-L-D).

  • 阳性对照
    • Human brain tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111367 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • 组织特异性Expressed in several normal tissues and human tumor-derived cell lines.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 发展阶段Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • 翻译后修饰Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c COT antibody
    • Cancer Osaka thyroid oncogene antibody
    • CCOT antibody
    • COT antibody
    • COT proto oncogene serine/threonine protein kinase antibody
    • EST antibody
    • ESTF antibody
    • Ewing sarcoma transformant antibody
    • FLJ10486 antibody
    • M3K8_HUMAN antibody
    • MAP3K 8 antibody
    • MAP3K8 antibody
    • MEKK8 antibody
    • Mitogen activated protein kinase kinase kinase 8 antibody
    • Mitogen-activated protein kinase kinase kinase 8 antibody
    • Proto oncogene cCot antibody
    • Proto-oncogene c-Cot antibody
    • Serine/threonine protein kinase cot antibody
    • Serine/threonine-protein kinase cot antibody
    • TPL 2 antibody
    • TPL-2 antibody
    • TPL2 antibody
    • Tumor progression locus 2 antibody
    see all

Anti-MAP3K8 (phospho S400) antibody 图像

  • ab111367 at 1/50 dilution staining MAP3K8 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phoshpopeptide.

Anti-MAP3K8 (phospho S400) antibody (ab111367)参考文献

ab111367 has not yet been referenced specifically in any publications.

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