Anti-MAP3K12抗体(ab37996)

概述

  • 产品名称Anti-MAP3K12抗体
    参阅全部 MAP3K12 一抗
  • 描述
    兔多克隆抗体to MAP3K12
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human MAP3K12 aa 828-859 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12852

  • 阳性对照
    • WB: HL-60 and SH-SY5Y cell lysates and mouse kidney tissue lysate. IHC-P: Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab37996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 95 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
  • 组织特异性Highly expressed in brain and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域Interacts with MBIP through the leucine-zipper motif.
  • 翻译后修饰Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
  • 细胞定位Cytoplasm. Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dlk antibody
    • Dual leucine zipper bearing kinase antibody
    • Dual leucine zipper kinase DLK antibody
    • Leucine-zipper protein kinase antibody
    • M3K12_HUMAN antibody
    • Map3k12 antibody
    • MAPK-upstream kinase antibody
    • MEKK12 antibody
    • Mitogen-activated protein kinase kinase kinase 12 antibody
    • Mixed lineage kinase antibody
    • MUK antibody
    • Protein kinase MUK antibody
    • ZPK antibody
    • ZPKP1 antibody
    see all

Anti-MAP3K12 antibody 图像

  • All lanes : Anti-MAP3K12 antibody (ab37996) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
  • All lanes : Anti-MAP3K12 antibody (ab37996)

    Lane 1 : HL-60 cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Secondary
    HRP-conjugated anti-rabbit IgG

    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,23 kDa,30 kDa,44 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MAP3K12 with ab37996. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

Anti-MAP3K12 antibody (ab37996)参考文献

ab37996 has not yet been referenced specifically in any publications.

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Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Spinal Cord)
Loading amount 20 µg
Specification Spinal Cord
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jan 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"