概述

  • 产品名称Anti-MAP2抗体[MT-01]
    参阅全部 MAP2 一抗
  • 描述
    小鼠单克隆抗体[MT-01] to MAP2
  • 经测试应用适用于: ICC, WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Cat, Pig
  • 免疫原

    Thermostable fraction of bovine brain microtubules.

性能

应用

Our Abpromise guarantee covers the use of ab7756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC
WB
IP
ICC/IF
  • 应用说明ICC: 1/200.
    ICC/IF: Use at an assay dependent dilution (PMID 18840692).
    IF: 1/200 - 1/1000.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
    • 序列相似性Contains 3 Tau/MAP repeats.
    • 翻译后修饰Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
    • 细胞定位Cytoplasm, cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686I2148 antibody
      • MAP 2 antibody
      • MAP dendrite specific antibody
      • MAP-2 antibody
      • MAP2 antibody
      • MAP2A antibody
      • MAP2B antibody
      • MAP2C antibody
      • Microtubule associated protein 2 antibody
      • Microtubule-associated protein 2 antibody
      • MTAP2_HUMAN antibody
      see all

    Anti-MAP2 antibody [MT-01] 图像

    • ab7756 at a dilution of 1/1000, staining MAP-2 (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images [A] and [B] show labeling of MAP-2 on cortical neuronal processes/axons using a 20x objective. [C] and [D] show labeling of MAP-2 in cortical axons observed with 40x objective. No labeling observed following omission of primary antibody.

      Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

    • ab7756 (1/200) immunostaining MAP2 protein in mouse cortical primary cell culture.

    实验方案

    Anti-MAP2 antibody [MT-01] (ab7756)参考文献

    This product has been referenced in:
    • Maghsoodi B  et al. Retinoic acid regulates RARalpha-mediated control of translation in dendritic RNA granules during homeostatic synaptic plasticity. Proc Natl Acad Sci U S A 105:16015-20 (2008). ICC/IF ; Mouse . Read more (PubMed: 18840692) »
    • Riederer BM  et al. Changes of MAP2 phosphorylation during brain development. J Histochem Cytochem 43:1269-84 (1995). Read more (PubMed: 8537643) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (spinal cord)
    Specification spinal cord
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 25 2008

    This antibody reacts with MAP2A and MAP2B form (tested on pig and mouse tissue samples. Reactivity with MAP2D on embryonal tissues has not been tested (reactivity was only tested on mouse cell line P19 in stage of neuronal differentionation). This ant...

    Read More

    This antibody has been tested and shown to cross react with cat, pig and mouse. Human has not yet been tested. I'll add this information to the datasheet.

    To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"